nitrocellulose membranes (Bio-Rad)

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Bio-Rad nitrocellulose membranes
Nitrocellulose Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trans+blot+nitrocellulose+membranes/Trans-Blot+Turbo+Mini+Nitrocellulose+Transfer+Packs/pmc13140060-235-24-26
Average 96 stars, based on 1302 article reviews

nitrocellulose membranes - by Bioz Stars, 2026-09

96/100 stars

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Western Blot:

Article Title: Polypeptides of
Article Snippet: Band migration was visualized using broad range kaleidoscope standards (BioRad) to aid in the electro-blot transfer while biotinylated broad range standards were used as molecular weight references on the blot, see FIGS. 7 and 8. .. For Western blot analysis, proteins were electroblotted from the gel onto trans-blot nitrocellulose membranes (BioRad) overnight, at 4° C. at 50 V, in Towbin buffer (25 mM Tris, 192 mM glycine, 20% methanol) using a BioRad Trans-Blot transfer cell and a Pac 300 power supply (BioRad). .. The nitrocellulose membrane was blocked using 3% fish gelatin (Sigma Chemical, St. Louis, Mo) in Tris buffered saline (TBS-20 mM Tris, 500 mM NaCl, pH 7.5) for 1 hour while shaking at 37° C. The membrane was dried at 37° C. and blocked in TBS containing 3% fish gelatin and this process was repeated.

Article Title: Proteins and immunizing compositions containing
Article Snippet: Band migration was visualized using broad range kaleidoscope standards (BioRad) to aid in the electro-blot transfer while biotinylated broad range standards were used as molecular weight references on the blot (see FIGS. 5A and 5B). .. For Western blot analysis, proteins were electroblotted from the gel onto trans-blot nitrocellulose membranes (BioRad) overnight, at 4° C. at 50 V, in Towbin buffer (25 mM Tris, 192 mM glycine, 20% methanol) using a BioRad Trans-Blot transfer cell and a Pac 300 power supply (BioRad). .. The nitrocellulose membrane was blocked using 3% fish gelatin (Sigma Chemical, St. Louis, Mo) in Tris buffered saline (TBS-20 mM Tris, 500 mM NaCl, pH 7.5) for 1 hour while shaking at 37° C. The membrane was dried at 37° C. and blocked in TBS containing 3% fish gelatin and this process was repeated.

Incubation:

Article Title: The Nematostella Wnt/β-catenin destruction complex provides insight into the evolution of the regulation of a major metazoan signal transduction pathway
Article Snippet: .. Protein gel was transferred onto Trans-Blot nitrocellulose membranes (Bio-Rad), the membranes were incubated with rabbit anti-mCherry antibody (1:1000) (PA5-34974, Invitrogen) or rabbit anti-flag antibody (1:1000) (cat# 740001, Invitrogen), and developed using goat anti rabbit IRDye 800 secondary antibodies (1: 10,000) (cat# C50331, LI-COR, Odyssey). .. Protein-protein interactions were detected between the following four pairs of proteins, NvAxin-flag x Nvβ-catenin-mCherry; SpAxin-flag x Spβ-catenin-mCherry; NvAxin-flag x Spβ-catenin-mCherry; SpAxin-flag x Nvβ-catenin-mCherry using the Pierce Crosslink Immunoprecipitation Kit with minor modifications (cat # 26147, Thermo Fisher Scientific, Waltham, MA).

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